excitatory neurons expressing Gcamp6f.  From Cris Niell's lab

Gcamp6f; pyramidal neurons; frontal cortex; mouse

V1 evoked responses

pupil and ball imaged using two separate Dalsa cameras that are triggered by the frame rate of the 2-photon scanning.

This video shows the raw data streams from two cameras: one imaging the ball and the other imaging the pupil.

Z-stack of 81 planes at 5um separation using knobby's z-stack function