This manual will help you understand the Neurolabware resonant scanning microscope, in order to minimize the amount of time, effort and additional complications during data acquisition.  As this is primarily an instrument for performing optical physiology and calcium imaging, we also hope we can aid in streamlining the analysis and interpretation of imaging data.

Topics will cover some imaging and two-photon excitation background, initial installation, hardware and optical maintenance and diagnostics, electronics diagnostics, upgrades, various assemblies, and some basic software functions.  A crucial parallel resource is Dario Ringach's ScanBox blog, which contains detailed procedures for specific tasks and calibrations involving the ScanBox software, various modular functions, and two-photon calcium imaging generally.